Literature Review of The Analytical Detection and Physiological Functions of Vitamin C

Abstract

Vitamin C (L-ascorbic acid) is an very important watersoluble vitamin with the highest daily requirement in human being, playing crucial physiological roles in immune regulation, antioxidant activity, collagen synthesis, and iron absorption. This paper systematically reviews several special properties of vitamin C with particular emphasis on analyzing the current research status of vitamin C detection technologies both domestically and internationally. These include traditional 2,6-dichlorophenolindophenol titration, ultraviolet spectrophotometry, and highperformance liquid chromatography, with comparative analysis of their respective advantages, disadvantages, and application scenarios. Furthermore, this paper addresses key controversies in current research, such as the “prooxidant” effect of vitamin C under specific conditions and the safety of long-term high-dose intake. Finally, future research directions are proposed, suggesting that detection technologies will evolve towards miniaturization and intelligence, functional studies will focus more on molecular mechanism exploration, and dietary strategies need to integrate innovative food engineering technologies, providing theoretical references for achieving precise nutrition and effective dietary supplementation of vitamin C.Furthermore,this paper discusses several controversies in contemporary research,including the “pro-oxidative”effect of vitamin C under specific conditions and the safety of long-term high-does intake.Finally,potential future research directions are proposed herein:detection techniques of vitamin C will advance toward miniaturization and intelligence ; functional studies will place greater emphasis on investigations into molecular mechanisms; and dietary strategies should integrate innovative food technologiesall of which aim to provide theoretical references for the realization of precision nutrition and effective dietary supplementation of vitamin C.